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Daniel Gladish
Professor Emeritus
Biological Sciences

Daniel Gladish

Contact Info

513-785-3244
Hamilton Campus, Mosler Hall 544

Education

  • Ph.D., Plant Biology, University of California, Davis
  • B.S., Botany, University of California, Davis

Biographical Information

In order to be prepared for the consequences of environmental change on wild and cultivated plants, it is important to understand how plants respond developmentally and physiologically to conditions in the environment.

In the past, my interests and research foci have been the effects of temperature on the development and physiology of root systems using physico-chemical techniques and computer image analysis. I completed a study that suggests the environment has influences on internal timing mechanisms (biological clocks) that regulate stage-specific developmental events beyond what can be attributed to simple thermodynamics.

More recently we have been studying the effects of flooding on root anatomy and development in legumes, especially vascular aerenchyma formation and programmed cell death.  We began evaluating the cell wall changes and gene expression associated with vascular aerenchyma formation.  We continued studies on the effects of temperature, flooding, and other environmental factors on the developmental anatomy and physiology of roots.  Most recently we have been using specially prepared and stained serial light microscope slides and computer tomography to virtually reconstruct root tips in various ways to reveal the histogenesis of particular tissues and tissue structural components to reevaluate classical theories of root growth and development.

Please note:  I retired in 2023, so I cannot sponsor or supervise any new graduate students.

Courses Taught

  • BIO/MBI 115: Biological Concepts
  • Bio 121: Environmental Biology
  • BIO 131: Plants, Humanity, and the Environment
  • BIO 171: Human Anatomy and Physiology
  • BIO 176: Ecology of North America
  • BIO 203: Introduction to Cell Biology
  • BIO 203L: Plant Cell Biology Laboratory
  • BIO 314: Plant and Fungal Diversity
  • BIO 402/502: Plant Anatomy

Selected Publications

  • Huang, Weiwei, Kehang Ma, and Daniel K. Gladish (2024). Ellipse or superellipse for tree-ring geometries? Evidence from six conifer species.  Trees doi.org/10.1007/s00468-024-02561-2.
  • Miki, Yasushi, Susumu Saito, Teruo Niki, and Daniel K. Gladish (2024). Re-evaluation of vascular histogenesis in the root tips of selected species in the Poaceae using new methods: analysis of the plerome, vascular initials, pericycle and late-maturing metaxylem vessels. Plants 13:910; https:// doi.org/10.3390/plants13060910.
  • Miki, Yasushi, Susumu Saito, Teruo Niki, and Daniel K. Gladish (2023).  Improved image processing for 3D virtual object construction from serial sections reveals tissue patterns in root tips of Zea mays.  Applications in Plant Sciences 8:e11531; doi:10.1002/aps3.11531.
  • Pegg, Timothy, Richard R. Edelmann, and Daniel K. Gladish (2020).  Immunoprofiling of cell wall carbohydrate modifications during flooding-induced aerenchyma formation in Fabaceae roots. Frontiers in Plant Science 10:1805; doi:10.3389/fpls.2019.01805.
  • Miki, Yasushi, Susumu Saito, Teruo Niki, and Daniel K. Gladish (2020).  3D digital image reconstruction of metaxylem vessels in root tips of Zea mays ssp mexicana (Poaceae) from transverse sections.  Applications in Plant Sciences 8:e11347; doi:10.1002/aps3.11347.
  • Saito, Susumu, Teruo Niki, and Daniel K. Gladish (2020).  Evaluation of metaxylem vessel histogenesis and the occurrence of vessel collapse during early development in primary roots of Zea mays ssp. mexicana: A result of premature programmed cell death?  Plants 9:374; doi:10.3390/plants9030374.
  • Saito, Susumu, Teruo Niki, and Daniel K. Gladish (2019).  Comparison of promeristem structure and ontogeny of procambium in primary roots of Zea mays ssp. mexicana and Z. mays ‘Honey Bantam’ with emphasis on metaxylem vessel histogenesis.  Plants 8:162; doi:10.3390/plants8060162.
  • Niki, Teruo, Susumu Saito, and Daniel K. Gladish (2019). A novel thin section preparation and staining protocol to increase contrast and resolution of cell details for light microscopy, Biotechnic and Histochemistry 94:522-526; doi:10.1080/10520295.2019.1601769
  • Pegg, Timothy J., Richard E. Edelmann, Daniel K. Gladish (2018).  Progression of cell wall matrix alterations during aerenchyma formation in Pisum sativum root cortical cells.  Microscopy & Microanalysis 24 (Suppl 1): 1378-1379.
  • Takahashi M, Niki T, Deem KD, Gladish DK (2016).  Vascular cavity formation enhances oxygen availability during flooding in root tips of Phaseolus coccineus L. primary roots.  International Journal of Plant Sciences (177:277-286).
  • Gladish DK (2015).  Vascular aerenchyma and PCD, in A. Gunawardena and P. McCabe, Eds., Plant Programmed Cell Death, Springer, Berlin.
  • Niki T, Saito S, Gladish DK (2015).  Granular bodies in root primary meristem cells of Zea mays L. var. Cuscoensis K. (Poaceae) that enter young vacuoles by invagination: a novel ribophagy mechanism.  Protoplasma 251: 1141-1149.
  • Sarkar P, Gladish DK (2012). Hypoxic Stress Triggers a Programmed Cell Death Pathway to Induce Vascular Cavity Formation in Pisum Sativum Roots. Physiologia Plantarum 146:413-426.
  • Niki T, Takahashi T, Gladish, DK (2011). Comparison of the effects of flooding vs. low-oxygen gas on pea (Pisum sativum L. cv. ‘Alaska’) primary roots. Plant Root 5:31-39.
  • Gladish DK, Niki T (2008). Ethylene is involved in vascular cavity formation in pea (Pisum sativum) primary roots. Plant Root 2: 38-45.
  • Sarkar P, Niki T, Gladish, DK (2008). Changes in cellular ultrastructure induced by sudden flooding at 25° C in Pisum sativum(Fabaceae) primary roots. American Journal of Botany 95:1-12.
  • Gladish DK, Xu J, Niki T (2006). Apoptosis-like programmed cell death occurs in procambium and ground meristem of pea (Pisum sativum) root tips exposed to sudden flooding. Annals of Botany 97: 895-902.
  • Niki T, Gladish, DK (2001). Changes in growth and structure of pea primary roots (Pisum sativum L. cv. ‘Alaska’) as a result of sudden flooding. Plant and Cell Physiology 42: 694-702.
  • Gladish DK, Sutter EG, Rost TL (2000). The role of free IAA levels, IAA transport, and sucrose transport in the high temperature inhibition of root system development in pea (Pisum sativum L. cv. Alaska). Journal of Plant Growth Regulation 19: 347-358.
  • Gladish DK, Niki T (2000). Factors inducing cavity formation in the vascular cylinders of pea roots (Pisum sativum L, cv. Alaska). Environmental and Experimental Botany 43: 1-9.