Anne Morris Hooke

Professor Emerita of Microbiology

Office: 32 Pearson Hall


Ph.D., Georgetown University 1979

Research Interests

Before I retired from Miami University, my interests were in the mechanisms bacteria use to infect human beings and cause disease, and how we can use this knowledge to prevent infection by bacterial pathogens. Specifically, our research exploited the properties of bacterial mutants that are unable to grow at body temperature and has been applied to the following three areas: the development of temperature-sensitive mutants of Escherichia coliPseudomonas aeruginosaSalmonella enteritidis and Listeria monocytogenes for use in quantitative assays measuring the bactericidal activity of phagocytes; the development of a model using temperature-sensitive mutants of E. coliP. aeruginosaBurkholderia cepaciaS. enteritidis and Haemophilus influenzae to study quantitatively the replication of the parental wild-types in experimental animals; and the combination of multiple temperature-sensitive mutations in a single strain to develop safe, live, attenuated vaccines against meningitis (H. influenzae), chronic Pseudomonas infection of patients with Cystic Fibrosis (P. aeruginosa), typhoid fever (S. typhi), strangles (Streptococcus equi) and swine pleuropneumonia (Actinobacillus pleuropneumoniae).

We also examined some of the extracellular products of the opportunistic pathogen B. cepacia, in particular the lipases responsible for hydrolysing egg yolk and a non-hemolytic phospholipase C (PLC). We purified and partially characterized the PLC and we studied its contribution to pathogenesis in a new model using the nematode, Caenorhabditis elegans.

Other projects in the laboratory included the identification and characterization of polyaromatic hydrocarbon degraders isolated from creosote-contaminated Superfund Sites, and the role of quorum sensing in the regulation of expression of the virulence factors produced by B. cepacia, A. pleuropneumoniae, and S. equi.

Selected Publications

  • Muhammad, N., and A. Morris Hooke. 2003. Biomass characterization of slow sand filtration schumutzdecke and its effects on filter performance. Environ. Technol. 24:43-50.
  • Muhammad, N., and A. Morris Hooke. 2003. Toxicity of heavy metals for microorganisms isolated from sand filter schmutzdecke. Environ. Technol. 24:1097-1102.
  • Weingart, C., and A. Morris Hooke. 1999. A nonhemolytic phospholipase C from Burkholderia cepacia. Curr. Microbiol. 38:233-238.
  • Weingart, C., and A. Morris Hooke. 1999. Regulation of expression of the nonhemolytic phospholipase C ofBurkholderia cepacia. Curr. Microbiol. 39:336-341.
  • Sordelli D. O., M.C. Cerquetti, M.C. Buzzola, V.E. Garcia, M.I. Gomez, and A. Morris Hooke. 1998. Attenuated mutants of Staphylococcus aureus with two temperature-sensitive lesions: isolation and characterization. MICROBIOS 94:95-102.