Facilities and Centers

Tracy Haynes at the microscope

Faculty and students conduct neuroscience research in laboratories in Pearson Hall and the Psychology Building. Both have numerous:

  • walk-in cold rooms
  • electrophoretic devices
  • fraction collectors
  • chromatographic equipment
  • ultraviolet visible spectrophotometers
  • homogenizers

Centrifugation equipment includes:

  • microcentrifuges
  • refrigerated low-speed and high-speed centrifuges
  • ultracentrifuges containing a variety of rotors accompanied by automatic density gradient fractionation equipment

Also available in Pearson Hall are:

  • microspectrophotometers
  • fluorescence microscopes
  • phase contrast microscopes

Also available in the Psychology Building are:

  • classical and instrumental conditioning chambers
  • In vivo electrophysiology recording apparati

Scintillation and gamma counters are available for research involving radioisotopes in a specially designed and equipped radioisotope laboratory.

Other Facilities and Centers

In addition to general laboratory facilities located in Pearson Hall and the Psychology Building, Miami University has several other state-of-the-art core facilities that are frequently used for Neuroscience research.

Animal Care Facility

Completed in 2009, this 13,000-ft2 facility includes specific pathogen-free transgenic animal suites, 2 surgical rooms and adjacent treatment and prep areas, conventional animal rooms, and a separate wing housing wild-caught species. The 2500-ft2 aquatics facility has 3 test rooms, 2 laboratories and 2 large culture rooms. Cage wash areas contain rack and cage washer, tunnel washer, as well as a pass-through autoclave. The staff includes 5 full-time employees plus student labor.

Bioinformatics and Functional Genomics, Center for (CBFG)

The Center for Bioinformatics and Functional Genomics at Miami University is a state of the art research and training facility that was recently renovated. The Center houses DNA sequencing and fragment analysis performed on 16 capillary ABI 3100 Genetic Analyzer and two ABI 310 Genetic analyzers. A Corbett Rotor-Gene 3000 thermocycler for RT and quantitative PCR, an Axon Gene-Pix 3000B DNA microarray scanner, digital gel documentation and analysis systems, thermocyclers and DNA fluorescence detectors, UV/VIS specs include NanoDrop microliter-scale spectrophotemeter, ELISA plate readers, and Molecular Dynamics Storm Phosphorimager. Directed by Dr. Luis Actis, Department of Microbiology.

Human Electrophysiology, Laboratory in

Located in the Psychology Building, the laboratory for human electrophysiology houses a dense array (256 channel)  electroencephalography (EEG) system with polygraphic input measures for traditional physiological responses that is intended to support both individual and interdisciplinary efforts with opportunities for industry collaboration.  Paradigms that can be implemented include computerized behavioral tasks as well as resting state protocols.  The facility is directed by Dr. Robin Thomas, Department of Psychology.

Microscopy and Imaging, Center for Advanced (CAMI)

The Center for Advanced Microscopy and Imaging features a Zeiss 710 laser scanning confocal microscope with seven laser lines and 37-channel spectral imaging housed in newly renovated, 5800 square foot core microscopy facility. Also, houses an Olympus FV500 laser scanning confocal microscope with seven laser excitation lines and 5-channel simultaneous acquisition. For electron microscopy a new (2009) JEOL JEM-2100 TEM/STEM with accelerating voltages of 70 to 200 kV, equipped with brightfield, darkfield, SAD, STEM, EFTEM imaging modes and magnifications from 500x - 1,500,000X. Several computers are available as workstations for image analysis using Image Pro Plus. Training and support are provided by Imaging Facility staff. 3-D reconstruction and analysis software are available. Facility located in Upham Hall and has 24 hour availability.

Tissue Culture Facility

A dedicated tissue culture facility consisting of several Forma Scientific® and NuAire® laminar flow tissue culture hoods, double-chamber Nuaire® water-jacketed CO2 incubators, a Hund/Wetzlar® Wilovert inverted microscope and freezers (-20 °C), refrigerators, water baths, bench-top centrifuges, and liquid N2 cell storage vessels. A separate room is dedicated to mouse embryonic stem cell culture, gene targeting and pre-implantation mouse embryo manipulation and transgenesis.