CAMI provides instruction and consultation to undergraduates, graduates, post-docs, faculty, and staff, in:
- transmission and scanning electron microscopy
- light microscopy
- digital imaging
Center for Advanced Microscopy and Imaging
The Center for Advanced Microscopy and Imaging (CAMI) is an all-university research, teaching, and service facility located in Upham Hall on Miami University's main campus, in Oxford, Ohio. CAMI serves the microscopy needs of both the biological and materials communities.
CAMI occupies 28 rooms with approximately 5400 ft2 of laboratory and lecture space. This space was newly renovated in 2010, and designed specifically to best meet the environmental needs for advanced microscopy. This renovation was also designed to provide the best integrated teaching and research space, and contains an interactive multi-media teaching/lecture lab for remote operation of the microscopes.
All of the laboratory rooms are climate controlled and the transmission electron microscope (TEM) lab spaces have building-independent humidity controls.
Our History
The microscopy center was established in 1987 as a shared facility through continued funding from the College of Arts and Science, the Graduate School, and a Department of Botany Academic Challenge Grant.
In 1996 the Electron Microscopy Facility (EMF) was administratively centralized under the College of Arts and Science. As the EMF diversified in both the disciplines it served and the types of microscopy it supported, the title of "Electron Microscopy" was no longer appropriate, and the facility was renamed as the Center for Advanced Microscopy and Imaging.
CAMI is used by members of numerous departments at Miami:
- Biology
- Chemical, Paper & Biomedical Engineering
- Chemistry and Biochemistry
- Geology & Environmental Earth Science
- Mechanical & Manufacturing Engineering
- Microbiology
- Physics
- Speech Pathology and Audiology
CAMI is used as well by researchers from a number of regional colleges and universities.
Associated Degree Programs
The Center for Advanced Microscopy & Imaging itself does not have a specific degree program. Students wishing to specialize in microscopy or ultrastructural imaging should pursue degrees (undergraduate or graduate) within specific academic programs at Miami. The CAMI works closely with faculty and students from members of the departments of:
Our Facilities and Equipment
Located on the lower level of Upham Hall, CAMI houses: 2 scanning electron microscopes (SEM), 2 transmission electron microscopes (TEM), 2 laser scanning confocal microscopes,1 deconvolution light microscope, 1 wide-field multimode light microscope, and 1 fluorescence stereoscope system.
Scanning Electron Microscopes (SEM)
This microscope uses electrons instead of light to form an image. Both SEMs are equipped with an energy-dispersive x-ray microanalysis (Bruker); one SEM has an electron back scattered diffraction system.
Transmission Electron Microscopes
The JEOL 2100 STEM has energy-dispersive x-ray microanalysis (Bruker), electron energy loss spectroscopy (Gatan EELS) and energy selective imaging (ESI Gatan GIF). All electron microscopes are covered under service contracts.
Instruction
Confocal Microscopy
Introductory and advanced training in confocal microscopy is provided on an as needed basis. In order to provide the best training, training is done on a one-on-one basis. If you need training in confocal microscopy contact Mr. Duley to arrange training.
Light Microscopy
Introductory and advanced training in light microscopy is provided on an as needed basis. In order to provide the best training, training is done on a one-on-one basis. If you need training in any type of light microscopy contact Mr. Duley to arrange training.
Special Instruction
Students are also able to create an independent course of study for variable credit hours in coordination with a CAMI staff member. Contact with the staff member and planning for the independent study course should be initiated during advance registration for the semester in which credit will be received.
Specialized personal instruction is also available from Matt l. Duley for computer usage, image analysis, confocal equipment, darkroom facilities, photography, general equipment, and advanced microscopy techniques. Contact him for more information.
Related Courses
This course provides a foundation in the principles and theories of microscopy with a major emphasis on transmission electron microscopy (TEM) and scanning electron microscopy (SEM). This course is designed to provide a theoretical background for students enrolled in the electron microscopy laboratory courses in the CAMI and for students not needing practical skills in EM but desiring a basis for understanding and evaluating ultrastructural research.
This laboratory course integrates the theory and practice of scanning electron microscopy (SEM), including sample preparation, SEM operation, darkroom work, manuscript preparation, and an individualized research project. This course provides hands-on experience from initial SEM specimen preparation, microscope usage and data collection, up to photographic plate preparation for publication. A wide variety of specimen preparation techniques are covered in order to best provide an understanding of the diversity of data collection possible via SEM. In addition to group laboratory sessions, students receive weekly individual instruction in the operation of the SEM.
This course provides hands-on experience in TEM specimen preparation, microscope operation, usage and data collection, and photographic plate preparation for publication. A wide variety of specimen preparation techniques are covered in order to illustrate the diversity of data collection possible via TEM. In addition to group laboratory sessions, students receive weekly individual instruction in the operation of the TEM during which they will produce micrographic images for evaluating the various specimen preparation techniques. This course is primarily designed for biological disciplines; however, modifications for material science students are available.
Co-Prerequisite: BIO 481/581, permission of instructor.
Personnel
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Zach Oestreicher,
