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A23 - Lasering in on Biological Tissues; Computational Modeling of Diffuse Reflectance and Fluorescence Spectroscopy for Tissue Sensing.
Mitochondrial metabolic processes are highly sensitive to thermal fluctuations.
A23 - Lasering in on Biological Tissues; Computational Modeling of Diffuse Reflectance and Fluorescence Spectroscopy for Tissue Sensing.
Mentor: Karthik Vishwanath, Ph.D.
Mitochondrial metabolic processes are highly sensitive to thermal fluctuations. To evaluate this relationship, we employed fluorescence spectroscopy to measure NADH autofluorescence in yeast cell cultures subjected to incremental heat stress. Readings were taken from yeast suspensions from a range of temperatures, along with the media without yeast and yeast with 0.15mL added ethanol. We found that increasing the temperature did not increase the NADH emission spectra on a statistically significant level, concluding that in the short term, increasing metabolic responses are due to increasing enzyme activity rather than increasing NADH biosynthesis.