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2025 Poster Session A
A06 - Tool development for the use of CRISPR to knockout TAT genes in cyanobacteria
Design a CRISPR construct to knockout TAT genes in cyanobacteria. Identify the optimal CRISPR plasmid backbone, design and insert the guide RNA (gRNA) into the plasmid, synthesize and insert repair templates with homology arms into plasmid, obtain final construct and transform in Cyanobacteria
2025 Poster Session A
A06 - Tool development for the use of CRISPR to knockout TAT genes in cyanobacteria
Mentor: Carole Dabney-Smith, Ph.D.
Design a CRISPR construct to knockout TAT genes in cyanobacteria. Identify the optimal CRISPR plasmid backbone, design and insert the guide RNA (gRNA) into the plasmid, synthesize and insert repair templates with homology arms into plasmid, obtain final construct and transform in Cyanobacteria