Atrazine is one of the most commonly used herbicides in the United States and is frequently found in freshwater ecosystems due to agricultural runoff. Its persistence in aquatic systems raises concerns about its impact on aquatic life, particularly during sensitive stages of development. This study examines the effects of short-term atrazine exposure on zebrafish (Danio rerio) embryos at specific developmental stages. Embryos were exposed to atrazine concentrations of 0.3 ppb, 3 ppb, and 30 ppb for 2 hours at four critical time points after fertilization (1–2 hpf, 6 hpf, 10 hpf, and 24 hpf). The primary outcomes measured were survival rates and hatching success. The results indicate that the effects of atrazine are stage-specific, with increased mortality and reduced hatching rates observed at the vulnerable developmental periods 6 hpf and 10 hpf. These findings support the hypothesis that even brief exposures to environmentally relevant concentrations of atrazine can have a negative impact on zebrafish embryonic development.