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Novel leiomodin 2b knockout in zebrafish as a model for dilated cardiomyopathy
Congenital heart disease (CHD) is highly prevalent in live births and requires concise diagnoses and treatment plans.
Novel leiomodin 2b knockout in zebrafish as a model for dilated cardiomyopathy
Mentor: Jennifer Schumacher, Ph.D.
Congenital heart disease (CHD) is highly prevalent in live births and requires concise diagnoses and treatment plans. Although there is a vast amount of genetics research in the field of CHD, there are still knowledge gaps regarding the gene networks surrounding heart development. A literature review revealed that mutation in Leiomodin 2 is associated with dilated cardiomyopathy in humans. We selected lmod2b for further molecular analysis. In situ hybridization was conducted throughout zebrafish embryonic development to investigate the expression pattern of lmod2b. To further understand the function of lmod2b, CRISPR-Cas9 gene knockout was conducted to look for cardiac abnormalities.