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General Laboratory Safety

Fundamental Concepts of Laboratory Chemical Safety

“Safety is essential when working with hazardous chemicals. This module covers the fundamentals of laboratory chemical safety, including the various roles and responsibilities for maintaining a safe laboratory workplace, the importance of working safely in laboratories, the documents and materials that are needed before work begins, and the requirements for laboratory safety training” (CITI Program, n.d.). Click here for instructions on how to access this training opportunity.

Chemical Storage and Segregation

“Proper chemical storage and segregation helps minimize potential exposures through leaks, spills, or reactions between incompatible materials. This module provides basic guidance on how to store chemicals according to their compatibility groups and hazard classes” (CITI Program, n.d.). Click here for instructions on how to access this training opportunity.

Emergency Planning

“Laboratory personnel must be able to prepare for, respond to, and recover from emergencies such as chemical spill, floods, fires, or severe weather. This module explains the four phases of emergency management, how to assess emergencies that can occur in a laboratory, and steps to follow when different emergencies occur” (CITI Program, n.d.). Click here for instructions on how to access this training opportunity.

Chemical Fume Hoods

“The chemical fume hood is a critical engineering control that helps protect laboratory personnel from hazardous chemical exposures. This module describes the chemical fume hood’s basic design functions and operations, the various types of chemical fume hoods and their specific uses, and how to implement safe work practices for chemical fume hood use” (CITI Program, n.d.). Click here for instructions on how to access this training opportunity.

Hazard Identification and Risk Assessment

“All laboratory experiments present different hazards and risks that must be continually identified, evaluated, controlled, and reviewed. This module discusses the importance of hazard identification and risk assessment, the key information sources that guide hazard identification and risk assessment, the different methods for controlling hazards, and the various health effects of laboratory chemicals” (CITI Program, n.d.). Click here for instructions on how to access this training opportunity.

Personal Protective Equipment (PPE) When Using Hazardous Chemicals

“Personal protective equipment is the “last line of defense” in the hierarchy of controls. It should be used in addition to other administrative, engineering, substitution, and elimination controls. This module explains the U.S. federal regulations and employer responsibilities regarding PPE, as well as chemical compatibility considerations, PPE that is necessary in the laboratory, and procedures for wearing and removing PPE” (CITI Program, n.d.). Click here for instructions on how to access this training opportunity.

Compressed Gas Cylinders

“Compressed gas cylinders can pose a significant risk to laboratory personnel because they can present chemical and physical hazards. This mishandling of compressed gas cylinders can result in disastrous consequences, including asphyxiation, fires and explosions, crush injuries, chemical exposures, and injuries from projectiles. This module explains the special hazards associated with compressed gas cylinders, the various types of compressed gas cylinders, the proper transport and safe storage of compressed gas cylinders, and the proper disposal of empty compressed gas cylinders” (CITI Program, n.d.). Click here for instructions on how to access this training opportunity.

Hazard Communication

“Compliance with OSHA 1910.1200 Hazard Communication Standard is essential to keep personnel informed and protect them from any health and physical hazards that may result when exposed to hazardous chemicals. This module will help personnel understand the key elements of hazard communication and how they apply in their workplaces” (CITI Program, n.d.). Click here for instructions on how to access this training opportunity.

Biological Safety

Basic Introduction to Biosafety

“Basic Introduction to Biosafety provides an introduction to biosafety for researchers handling Risk Group 1 agents and working only at Biosafety Level 1 (BSL-1) containment” (CITI Program, n.d.). Click here for instructions on how to access this training opportunity.

OSHA Bloodborne Pathogens

“OSHA Bloodborne Pathogens is designed as initial training or annual retraining to meet the requirements of the U.S. Occupational Safety and Health Administration’s (OSHA) Bloodborne Pathogen Standard” (CITI Program, n.d.). Click here for instructions on how to access this training opportunity.

NIH Recombinant DNA Guidelines

“NIH Recombinant DNA Guidelines provides principal investigators and those responsible for the conduct of recombinant and synthetic nucleic acid research experiments with basic training and an overview of the requirements established by the National Institutes of Health (NIH)” (CITI Program, n.d.). Click here for instructions on how to access this training opportunity.

Dual Use Research of Concern

“Dual Use Research of Concern (DURC) offers detailed training on the United States Government Policy for Oversight of Dual Use Research of Concern Pathogens with Enhanced Pandemic Potential. The course is based on the 2024 policy and focuses on research oversight to increase the awareness of biosafety and biosecurity and the implementation of risk mitigation. The U.S. Government Policy for Oversight of Dual Use Research of Concern and Pathogens with Enhanced Pandemic Potential applies to research from the proposal stage through funding, research activities, and publication” (CITI Program, n.d.). Click here for instructions on how to access this training opportunity.

Fundamentals of Working Safely in a Biological Safety Cabinet (BSC)

“A biological safety cabinet (BSC) is the primary means of containment developed for working safely with infectious microorganisms. Class II BSCs, the most common cabinets used in laboratories, are designed to provide personnel protection, product protection, and environmental protection. This basic-level eLearning course provides information on the safe use of Class II biological safety cabinets. Topics covered include major parts of a BSC, how a BSC works, how to work safely inside a BSC, and what to do if there is an emergency while working in a BSC” (U.S. Centers for Disease Control and Prevention, n.d.). Click here for instructions on how to access this training opportunity.

Viral Vectors

The viral vector training modules, from the University of Cincinnati, provide information pertaining to risk assessment and address lentiviral vectors, gamma-retroviral vectors, adenoviral vectors, and adeno-associated (AAV) vectors. Click here for instructions on how to access this training opportunity.

Special Topics

Laser Safety

“This course provides fundamental training for the use of lasers and laser systems in research and medical institutions. A crucial piece of every laser safety program is the knowledge, by each individual working with or near lasers, of the hazards faced and the ways in which the work can be performed safely. It includes a review of basic laser concepts, specific types of lasers and their uses, hazard evaluations and classifications, potential biological effects and mechanisms of injury, safety practices and protective measures, and regulations” (CITI Program, n.d.). Click here for instructions on how to access this training opportunity.

Fundamentals of Methylene Chloride Safety

“This basic-level course provides information on the chemical properties and adverse health effects associated with the use of methylene chloride, or dichloromethane (DCM), in clinical and public health laboratories” (U.S. Centers for Disease Control and Prevention). Click here for instructions on how to access this training opportunity.

Training Opportunities

CITI Program

CITI Program offers a variety of training courses, including laboratory safety and biosafety.

Viral Vectors

University of Cincinnati offers training modules for the safe use of viral vectors, such as lentiviral vectors, gamma-retroviral vectors, adenoviral vectors, and adeno-associated viral vectors.

Fundamentals of Methylene Chloride Safety

This course, offered by the U.S. Centers for Disease Control and Prevention, provides users with an overview of key safety considerations when utilizing methylene chloride.

References

CITI Program. (n.d.). Basic Introduction to Biosafety. Basic Introduction to Biosafety | CITI Program

CITI Program. (n.d.). Dual Use Research of Concern (DURC). Dual Use Research of Concern (DURC) | CITI Program

CITI Program. (n.d.). Laboratory Chemical Safety. Laboratory Chemical Safety | CITI Program

CITI Program. (n.d.). Laser Safety. Laser Safety | CITI Program

CITI Program. (n.d.). NIH Recombinant DNA Guidelines. NIH Recombinant DNA Guidelines | CITI Program

CITI Program. (n.d.). OSHA Bloodborne Pathogens. OSHA Bloodborne Pathogens | CITI Program

U.S. Centers for Disease Control and Prevention. OneLabREACH: Fundamentals of Methylene Chloride Safety. Fundamentals of Methylene Chloride Safety | OneLab REACH

U.S. Centers for Disease Control and Prevention. OneLabREACH: Fundamentals of Working Safely in a Biological Safety Cabinet (BSC). Fundamentals of Working Safely in a Biological Safety Cabinet (BSC) | OneLab REACH